Application

Research CategoryNeuroscience

Detect Dopamine D3 Receptor using this Anti-Dopamine D3 Receptor Antibody, cytoplasmic domain validated for use in ELISA, IH & WB.

Research Sub CategoryNeurotransmitters & Receptors

Western blot: 1-10 µg/mL (Chemiluminescence technique) Approximately 100-400 µg of membrane protein/lane should be loaded to visualize the receptor band.

Immunohistochemistry: 1-20 µg/mL on paraformaldehyde fixed sections.

ELISA: 0.5-1.0 µg/mL (1 µg/mL immunogen peptide (Cat. Number AG224)/well)

Optimal working dilutions must be determined by the end user.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Epitope: cytoplasmic domain

A 19 amino acid peptide sequence from the human D3 receptor within the 3rd cytoplasmic domain.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Affinity purified immunoglobulin. Liquid in PBS, pH 7.5 with 0.1% BSA. No preservatives

Specificity

Specific for Dopamine Receptor D3. Human Dopamine D3 receptor is a 400 aa, G-protein coupled and transmembrane receptor protein (Giros et al. 1990, 1991; Schmauss et al. 1993; Liu et al. 1994; Fu et al. 1995; Sokoloff et al. 1990; Livingstone et al. 1992). The immunogen peptide shows no significant sequence similarity with other dopamine receptors (D1, D2, D4 or D5).

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.